Solid-State Chromatin Immunoprecipitation

Chromatin immunoprecipitation (ChIP) with the patented bead-free technology from Chromatrap® provided from Porvair Sciences utilizes filters with an inert porous polymer matrix coupled with Protein-A or Protein-G on the surface. This increased surface area maximizes immunocapture of antibody-chromatin complexes from your samples.

Features:

  • Easy to use and simple spin column protocol with less manual handling
  • Highest sensitivity and binding capacity through optimally oriented Protein-A/G arrangement across filter (see Figure)
  • No sample loss as antibody-chromatin complexes are captured and retained on filter
  • Lowest background and exceptional signal-to-noise ratio compared to other ChIP kits
  • Lowest amount input material, highest DNA yield
  • ChIP from as low as 1000 cells, 50 ng chromatin or 5 mg tissue
  • Availabe as Protein-A/G spin columns or 96 well filter plates
  • Chromatin-to-Sequencing ready in under 5 hours
  • Applications: Next Generation Sequencing, PCR, RT-PCR, Mass Spectrometry 

Antibodies and Primers for ChIP assays

  • Use of high-quality, specific ChIP validated antibody is essential for the success of a ChIP assay
  • Positive and negative control targets
  • Can be supplied with primer sets for extra validation
  • Reduce time spent searching databases and designing primers
  • Confidence in correct targets for antibodies

 ChIP-Seq Kits

  • Most sensitive kit at low cell numbers
  • Can be used with:
    • Cell lines, primary cells, and difficult cell types and tissues
    • Low abundant targets
    • Library preparation kits
    • All sequencing platforms (e.g. Inc Illumina,              Ion torrent, Roche)

 ChIP-Seq HT 96-well Plates


  • Perform up to 96 reactions simultaneously in 1 day 
  • Pair with automated liquid handling for even higher throughput
  • Can be used for:
    • Antibody validation 
    • Multiple antibodies 
    • Multiple samples 
    • Multiple cell types
    • Multiple drug compounds/inhibitors

 UniqSeq Kits


  • Complete solution for ChIP and library preparation for next generation sequencing
  • Compatible with all Illumina sequencers 
  • Low levels of duplication (< 2 %)
  • High (98 %) uniquely mapped reads 
  • One tube reaction – End repair, A-tail & ligation in one step
  • Bead free size selection 
  • Low input (500 pg DNA)

 Specialised ChIP Kits – Native

  • Only commercial method to study chromatin in its    ‘native’ state 
  • No chemical fixation required 
  • Cells are in more natural ‘native’ state 
  • Can be used for:
    • Non-fixed samples
    • Histone modifications
    • Transcription factors tightly associated with DNA

Specialised ChIP Kits – FFPE

  • For use with formalin fixed paraffin embedded (FFPE) tissue 
  • Can be used for: 
    • Tissue samples
    • Biopsies
    • Difficult-to-lyse samples
    • Range of different FFPE tissue (human & animal)

 Specialised ChIP Kits – Drosophila

  • Only commercial method to study Drosophila (fruit fly) chromatin
  • Focus on Drosophila community of researchers and neuroscientists 
  • Uses as little as 5 flies or 500 ng chromatin 
  • Can be used for: 
    • Whole body samples
    • Larvae samples 
    • Pupal samples
    • Embryonic samples 
    • Histone modifications
    • Transcription factors

Shearing Kits

 For optimisation of shearing conditions 

-->Sonication
  • Simple and effective method for preparation of high-quality chromatin
  • Can be used for most cell types and tissues 
  • Useful for difficult to lyse samples
-->Enzymatic
  •  Suitable for native chromatin preparation
  •  Effective alternative to sonication as no expensive       equipment is required
  •  Milder treatment, can be less disruptive to the epitopes 

 

gDNA Removal prior to RNA Extraction

The new Chromatrap® genomic DNA (gDNA) Removal Columns can be used to eliminate gDNA along with RNA extraction recommended with Chromatrap® RNA Extraction kit. This procedure ensures maximum purity and yield of pure RNA from a wide range of samples in a fast and cost-effective way. 

Features of Chromatrap® gDNA Removal Columns:
  • Enzyme-free gDNA removal from cell lysates within 30 seconds / sample
  • Load and spin method for gDNA-free, total RNA
  • Simple, one-step centrifugation method
  • Highly specific gDNA removal with no loss of precious RNA 
  • Ideal for DNA-sensitive applications
  • Can be used as an upstream gDNA removal kit prior to RNA extraction
Features of Chromatrap® RNA Extraction kit:
  • Reproducible and fast extraction of high-quality total RNA
  • Compatible with a wide range of samples including buffy coat, buccal swabs, and gram-negative bacteria
  • Process up to 107 cultured animal cells, 50 mg animal tissue, 0.5 ml blood, 250 mg plant material, 5 x 107 yeasts or 5 x 108 bacteria  
  • High yield extraction of up to 1000 µg pure RNA
  • Application-ready total RNA for qPCR, Microarray or other in vitro applications 

Chromatrap® DNA Extraction

  • Used to extract DNA from cells, tissues and blood samples 
  • Allows highest amount of DNA recovery from starting sample (DNA recovery up to 98 %)
  • Purification of up to 50 µg DNA 
  • Samples are free from contaminants such as salts, inhibitors or enzymes
  • For applications such as PCR, ChIP, Southern blotting, DNA methylation studies, Molecular cloning and Sequencing 
  • Phenol- and chloroform-free

DNA kits to extract, purify, 
concentrate and size select

  • Simple and fast procedure
  • In 96-well format for high-throughput
  • DNA Extraction: high quality DNA from up to 25 mg tissue or 5 million cells in 200 µl
  • DNA Purification: removal of undesired impurities from up to 50 µg DNA
  • DNA Purify & Concentrate: Purify and concentrate up to  10 µg DNA in as little as 5 to 10 µl
  • Size Selection: Bead-free exclusion of DNA fragments        < 200 bp

Chromatrap® 
Homogeniser Spin Columns

Quickly and efficiently homogenise cells and tissues lysates
in a single step. Contained within a spin column, the unique
proprietary bio-shredding Vyon® polymer reduces lysate viscosity and captures insoluble debris by centrifugation.

  • Perfect alternative to syringe & needle homogenisation
  • Quick, clean and efficient homogenisation
  • Reduces viscosity of lysate
  • Ideal sample preparation for nucleic acid miniprep and midiprep
  • Manufactured from PP, stable until -80 °C
  • Centrifuge at max. 25,000 x g with fixed angled rotors and 30,000 x g with swing out rotors
  • Maximal sample volume of 700 µl
  • No cross contamination possible

Weitere Informationen zu den Produkten von Porvair Sciences können Sie hier finden:

- Geräte

- Verbrauchsmaterialien